[GANN, 58, 221-227; June, 1967] UDC 616.381-003.217-006.3-092.4:612.015.3[547.455.6] METABOLISM OF GLUCOSE IN YOSHIDA SARCOMA AND ITS SLOW-GROWING VARIANT SUBLINES*1

The three slow-growing variant sublines of Yoshida sarcoma, LY-7, LY-5, and LY-336, as compared with the parent tumor, showed a marked increase in activity to synthesize glycogen from added glucose in vitro. The activity increases roughly parallel with the increasing survival time of the tumors. The glyconeogenetic activity of Yoshida sarcoma, as determined by the incorporation of pyruvate-carbon into glycogen, was enhanced on the addition of glucose and depressed by the presence of an inorganic phosphate. Under the conditions employed, LY-336, the slowest-growing tumor, showed a greater glyconeogenetic activity than any of the other three tumors. No significant difference was observed in the glycolytic and glucose-oxidizing activities of the four strains of the tumor. The possible significance of these findings is discussed in relation to the biological characteristics of these variant-sublines.